ChloroquineV1, Main, Exploration, bibRecord, 002909

The reduction of the positive charges of polylysine by partial gluconoylation increases the transfection efficiency of polylysine/DNA complexes

Identifieur interne : 002909 ( Main/Exploration ); précédent : 002908; suivant : 002910

The reduction of the positive charges of polylysine by partial gluconoylation increases the transfection efficiency of polylysine/DNA complexes

Auteurs : Patrick Erbacher [France] ; Annie Claude Roche [France] ; Michel Monsigny [France] ; Patrick Midoux [France]

Source :

BBA - Biomembranes [ 0005-2736 ] ; 1997.

RBID : ISTEX:D7ACD0DAE2EEB15A4EF5F8B9A2B7A73CD7B7616A

English descriptors

KwdEn :
- Chloroquine, DP, degree of polymerization, FBS, fetal bovine serum, Gene therapy, Gene transfer, GlcA, gluconoyl residue, Lact, lactosyl residue, PBS, phosphate-buffered saline, pH 7.4, Polylysine, pLK, poly-l-lysine..
Teeft :
- Acta, Average number, Biochimica, Biol, Biophysica, Biophysica acta, Cell lines, Chloroquine, Complete retardation, Complexed, Dmem, Electrostatic interactions, Erbacher, Gene expression, Gene transfer, Gibco, Glca, Glca molecules, Glca residues, Glcaplk, Glcaplkrdna, Gluconoyl, Gluconoyl residues, Gluconoylated, Gluconoylated polylysine, Grml, Hepg2, Hepg2 cells, Humidified atmosphere, Lact, Lactosyl, Lactosyl residues, Lactosylated, Lactosylated polylysine, Lowest polymer, Luciferase, Luciferase activity, Midoux, Molar, Molar ratio, Monsigny, Negative charges, Plasmid, Plasmid complexed, Plkrpsv2, Pmol, Polylysine, Polymer, Polymerrdna, Polymerrdna molar ratio, Positive charges, Psv2, Recognition signals, Small number, Transfected, Transfection, Transfection efficiency.

Abstract

Abstract: A polylysine partially substituted with polyhydroxyalkanoyl residues and specially with gluconoyl residues was developed in order to increase the transfection efficiency by decreasing the strength of the electrostatic interactions between the DNA and the cationic polymer. Partially gluconoylated polylysine/DNA complexes were more easily dissociated in solution and their transfection efficiency in the presence of chloroquine, evaluated with HepG2 cells, a human hepatocarcinoma line, was higher when 43±4% of the ϵ-amino groups of polylysine were blocked with gluconoyl residues. Partially gluconoylated polylysine/plasmid complexes were efficient in transfecting different adherent as well as non-adherent cell lines. Partially gluconoylated polylysine formed highly soluble (above 100 μg/ml in DNA) complexes with DNA plasmids. In addition, partially gluconoylated polylysine bearing few lactosyl residues increased the transfection efficiency of HepG2 cells which express a galactose-specific membrane lectin.

Url:

DOI: 10.1016/S0005-2736(96)00204-0

Affiliations:

Le document en format XML

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<term>Gene transfer</term>
<term>GlcA, gluconoyl residue</term>
<term>Lact, lactosyl residue</term>
<term>PBS, phosphate-buffered saline, pH 7.4</term>
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<term>Biophysica</term>
<term>Biophysica acta</term>
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<term>Chloroquine</term>
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<term>Complexed</term>
<term>Dmem</term>
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<front><div type="abstract" xml:lang="en">Abstract: A polylysine partially substituted with polyhydroxyalkanoyl residues and specially with gluconoyl residues was developed in order to increase the transfection efficiency by decreasing the strength of the electrostatic interactions between the DNA and the cationic polymer. Partially gluconoylated polylysine/DNA complexes were more easily dissociated in solution and their transfection efficiency in the presence of chloroquine, evaluated with HepG2 cells, a human hepatocarcinoma line, was higher when 43±4% of the ϵ-amino groups of polylysine were blocked with gluconoyl residues. Partially gluconoylated polylysine/plasmid complexes were efficient in transfecting different adherent as well as non-adherent cell lines. Partially gluconoylated polylysine formed highly soluble (above 100 μg/ml in DNA) complexes with DNA plasmids. In addition, partially gluconoylated polylysine bearing few lactosyl residues increased the transfection efficiency of HepG2 cells which express a galactose-specific membrane lectin.</div>
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The reduction of the positive charges of polylysine by partial gluconoylation increases the transfection efficiency of polylysine/DNA complexes

The reduction of the positive charges of polylysine by partial gluconoylation increases the transfection efficiency of polylysine/DNA complexes

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

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